2SP (SUCROSE-PHOSPHATE) CHLAMYDIA TRANSPORT MEDIUM

Technical Data #2447KRa / 2013.06.05

2SP Chlamydia Transport Medium is a selective medium used to transport specimens contaminated with Chlamydia sp. Antibiotics prevent bacterial overgrowth and phenol red is the indicator. Fetal calf serum is provided as the nutrious ingredient.

 


 

FORMULA
in grams per liter purified filtered water

Sucrose

68,5

Potassium Phosphate, dibasic

2,07

Potassium Phosphate, monobasic

1,1

Phenol Red

0,01

Fetal Calf Serum

50 ml

Streptomycin

0,05

Vancomycin

0,1

Nystatin

25 000 U

pH 7,0 +/- 0,2 à 25º C

This approximate formula may be adjusted and/or enriched to obtain best results.


PRECAUTIONS
This medium is for IN VITRO diagnostic use only.


STORAGE
Store prepared media at -20º C or at 2-8º C protected from direct light.


SIGN OF DETERIORATION
Media should not be used if passed the expiration date. Prepared media should not be used if there are signs of contamination or deterioration (evaporation or discoloration).


SPECIMEN COLLECTION

Cervical Samples

  1. Remove mucus from exocervix with a large swab and discard.
  2. Insert other large swab into endocervical canal until tip is no longer visible.
  3. Rotate 3-5 seconds. Withdraw. avoid contact with vaginal surfaces.

Urethral Samples

  1. Avoid urination for 1 hour prior to sampling.
  2. Insert small swab 2-4 cm into urethra.
  3. Rotate 3-5 seconds. Withdraw..


SPECIMEN TRANSPORT

  1. Place swab in tube and break at score line.
  2. Bring as soon as possible to the laboratory. The chlamydial specimens should be refrigerated at 2-8º C if processed within 24 hours after collection. If not, freeze at -60º C.
  3. Follow the recommended procedures for primary isolation of chlamydiae.


REFERENCES

  1. Cles, L.D., K. Bruch, W.E. Stamm 1988 Staining characteristic of six commercially available monoclonal immunofluorescence reagents for direct diagnosis of Chlamydia trachomatis infections. J. Clin. Microbiol. 26:1735-1737.
  2. Clyde W.A., G.E. Kenny, J. Schachter, 1984 Laboratory diagnosis of chlamydial and mycoplasmal infections. Cumitech 19 American Society for Microbiology
  3. Gordon, F.B., I.A. Harper, A.L. Quan, J.D. Treharne, R.S.C. Dwyer, J.A. Garland 1969 Detection of Chlamydia (Bedsonia) in certain infections of man. I. Laboratory procedures: comparaison of yolk sac and cell culture for detection and isolation. J. Infect. Dis. 120:451-462.
  4. Kuo, C.C., J.T. Grayston 1988 Factors affecting viability and growth in HeLa 229 cells of Chlamydia sp. strain TWAR. J. Clin. Microbiol. 26:812-815.
  5. Lennette, E.H., A. Balows, W.J. Hausler Jr., H.J. Shadomy. 1985 Manual of Clinical Microbiology. 4th ed. Washington D.C.: American Society for Microbiology.
  6. Stamm, W.E., M. Tam, M. Koester, L. Cles 1983 Detection of Chlamydia trachomatis inclusions in McCoy cell cultures with fluorescein-conjugated monoclonal antibodies. J. Clin. Microbiol. 17:666-668.
  7. Yoder, B.L., W.E. Stamm, M.C. Koester, E.R. Alexander 1981 Microtest procedure for isolation of Chlamydia trachomatis J. Clin. Microbiol. 13:1036-1039.


CATALOGUE NUMBERS

Prepared media    
Tubes, 16x125 mm, 1 ml 10/pkg 2550
Tubes, 16x125 mm, 2 ml 10/pkg 2837
     
Collection and Transport Kit

Contents:
3 Sterile Swabs (2 Large, 1 Small), 1 2SP Transport Medium, 13x100 mmm, 2 ml
  2447-KR

 


 

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