MUELLER HINTON BROTH

Technical Data #2450a / 2013.06.30

Mueller Hinton Broth is a liquid general purpose medium for the cultivation of a large quantity of bacteria. This medium may be used for for the antimicrobial susceptibility by the agar diffusion disc or by dilution methods.

 


 

FORMULA
in grams per liter purified filtered water

Beef Extract

2,0

Casein Hydrolysate, acid

17,5

Starch

1,5

pH 7,3 +/- 0,2 à 25º C

This approximate formula may be adjusted and/or enriched to obtain best results.


PRECAUTIONS
This medium is for IN VITRO diagnostic use only.


STORAGE
Store prepared media at 2-8º C protected from direct light and dehydrated powder, in a dry place in tightly-sealed containers at 2-25º C.


SIGN OF DETERIORATION
Media should not be used if the expiry date has passed. prepared media should not be used if there are signs of contamination (evaporation or discoloration). Do not use dehydrated media if it is caked.


DIRECTIONS
Suspend 21 g in 1000 ml of purified water. Heat with frequent agitation and boil for one minute. Mix thouroughly and dispense into culture tubes. Sterilize at 121º C for 15 minutes.


PROCEDURE
Bring prepared medium at room temperature before inoculation.

  1. Inoculate according to previous standardized procedures.
  2. Incubate at 35º C for 24-48 heures with loosened caps.
  3. Examine for growth.


QUALITY CONTROL

Expected results after 24 hrs at 35ºC

Organisms

ATCC

Growth

Escherichia coli

25922

+

Staphylococcus aureus

25923

+

Pseudomonas aeruginosa

27853

+

Streptococcus faecalis

29212

+

Neisseria meningitidis

13090

+


LIMITATIONS OF METHOD
This is only a part of the identification. Others tests may be required.


REFERENCES

  1. Lennette, E.H., Ballows, A., Hausler, W.J.Jr., and Shadomy, H.J. Manual of Clinical Microbiology. 4th ed. 1985 Washington D.C.: American society for Microbiology.
  2. Mac Faddin, Jean F. 1985 Media for Isolation-Cultivation-Identification-Maintenance of Medical Bacteria. Vol. I, Baltimore, MD.: Williams & Wilkins.
  3. NCCLS 1990 Performance standard for antimicrobial disc susceptibility tests Approved Standard. Document:M2-A4 Vol. 10, No. 7


CATALOGUE NUMBERS

Dehydrated media 500 g QB-39-3208
     
Prepared media    
Tubes 13x100 mm, 2 ml 10/pkg 2450
Tubes 16x125 mm, 5 ml 10/pkg 2663
Bottles 5 oz 100 ml 6487

 


 

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