B.H.I. (BRAIN HEART INFUSION) BROTH

Technical Data #2475a / 2013.07.01

BHI Broth is a an highly nutrient general purpose medium for the cultivation of fastidious microorganisms, such as pneumococci, streptococci and meningococci.

 


 

FORMULA
in grams per liter purified filtered water

Casein Peptone

15,0

Meat Peptone

6,0

Brain Heart Infusion

6,0

Yeast Extract

5,0

Dextrose

2,0

Sodium Chloride

5,5

Potassium Phosphate, dibasic

2,5

pH 7,4 +/- 0,2 à 25º C

This approximate formula may be adjusted and/or enriched to obtain best results.


PRECAUTIONS
This medium is for laboratory use only.


STORAGE
Store prepared media at 2-8º C protected from direct light and dehydrated powder, in a dry place in tightly-sealed containers at 2-25º C.

SIGN OF DETERIORATION
Media should not be used if the expiry date has passed. prepared media should not be used if there are signs of contamination (evaporation or discoloration). Do not use dehydrated media if it is caked.


DIRECTIONS
Suspend 42 g in 1000 ml of purified water. Heat with frequent agitation and boil for one minute. Sterilize at 121º C for 15 minutes. Cool to 45-50º C. Mix thouroughly and dispense into sterile culture tubes.


PROCEDURE
Bring prepared medium at room temperature before inoculation.

  1. Inoculate according to previous standardized procedures.
  2. Incubate at 35º C for 24 hours.
  3. Examine for growth.


QUALITY CONTROL

Expected results after 24 hrs at 35ºC

Organisms

ATCC

Growth

Escherichia coli

25922

+

Staphylococcus aureus

25923

+

Neisseria meningitidis

13090

+

Streptococcus pneumoniae

6305

+

Streptococcus pyogenes

19615

+


LIMITATIONS OF METHOD
This is only a part of the identification. Others tests may be required.


REFERENCES

  1. Lennette, E.H., Ballows, A., Hausler, W.J.Jr., and Shadomy, H.J. Manual of Clinical Microbiology. 4th ed. 1985 Washington D.C.: American society for Microbiology.
  2. Mac Faddin, Jean F. 1985 Media for Isolation-Cultivation-Identification-Maintenance of Medical Bacteria. Vol. I, Baltimore, MD.: Williams & Wilkins.
  3. N.C.C.L.S. 1990 Quality Assurance for Commercially Prepared Microbiological Culture Media. Approved Standard. Vol. 10, No. 14, NCCLS Document M22-A.


CATALOGUE NUMBERS

Dehydrated media 500 g QB-39-0305
     
Prepared media    
Tubes    
13x100 mm, 2 ml 10/pkg 2784
13x100 mm, 0,5 ml 10/pkg 2475
16x125 mm, 5 ml 10/pkg 2677
16x125 mm, 10 ml 10/pkg 2740
     
Bottles, 5 oz 10X100 ml 6614

 


 

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