MOTILITY TEST MEDIUM W/ TUBE ï MOTILITY TEST MEDIUM W/ T.T.C.

Technical Data #2496a / 2013.07.20

Motility Test Medium is a semisolid medium used in the detection of enteric pathogens motility. TTC addition shows a positive motility test if a diffused reddish color appears throughout the medium. A negative result shows a reddish line along the inoculation, only.

 


 

FORMULA
in grams per liter purified filtered water

Casein Peptone

5,0

Meat Peptone

5,0

Beef Extract

1,0

Sodium Chloride

5,0

Agar

4,0

T.T.C. (Triphenyltetrazolium Chloride)

0,05

pH 7.2 +/- 0,2 à 25º C

This approximate formula may be adjusted and/or enriched to obtain best results.


PRECAUTIONS
This medium is for IN VITRO diagnostic use only.


STORAGE
Store prepared media at 2-8º C protected from direct light and dehydrated powder, in a dry place in tightly-sealed containers at 2-25º C.


SIGN OF DETERIORATION
Media should not be used if the expiry date has passed. prepared media should not be used if there are signs of contamination (shrinking, cracking, evaporation or discoloration). Do not use dehydrated media if it is caked.


DIRECTIONS
Suspend 20 g in 1000 ml of purified water. Heat with frequent agitation and boil for one minute. Sterilize at 121º C for 15 minutes. Cool to 45-50º C. Add 5 ml of sterile 1% TTC solution (8589). Mix thouroughly and dispense into sterile culture tubes. For the Motility Test Medium with tube, dispense into sterile culture tube containing a two sides open tube.


PROCEDURE
Bring prepared medium at room temperature before inoculation.

  1. Inoculate with 2-3 similar colonies from an overnight growth, by stabbing with a straight needle to over half the depth.
  2. Incubate at 35º C for 24 heures, with loosened caps.


QUALITY CONTROL

Expected results after 24 hrs at 35ºC

Organisms

ATCC

Motility

w/TTC

Escherichia coli

25922

+

diffused red

Proteus mirabilis

12453

+

diffused red

Klebsiella pneumoniae

13883

-

red line

Shigella flexneri

12022

-

red line


LIMITATIONS OF METHOD
As TTC may be inhibitory to some bacteria a Motility Test with tube and without TTC should be inoculated and incubated at the same moment. This is only a part of the identification. Others tests may be required.


REFERENCES

  1. Finegold, S.M., E.J. Baron 1986 Bailey and Scott's Diagnostic Microbiology 7th ed. C.V. Mosby St. Louis
  2. Lennette, E.H., Ballows, A., Hausler, W.J.Jr., and Shadomy, H.J. Manual of Clinical Microbiology. 4th ed. 1985 Washington D.C.: American society for Microbiology.
  3. Mac Faddin, Jean F. 1985 Media for Isolation-Cultivation-Identification-Maintenance of Medical Bacteria. Vol. I, Baltimore, MD.: Williams & Wilkins.


CATALOGUE NUMBERS

Dehydrated media 500 g QB-39-3006
     
Prepared media    
Tubes 16x125 mm, w/ tube, 5 ml 10/pkg 2496
Tubes 16x125 mm, w/ tube, 10 ml 10/pkg 2696
Tubes 16x125 mm, w/ TTC, 5 ml 10/pkg 2977
Tubes 16x125 mm, w/ TTC, 7 ml 10/pkg 2649
     
Sterile 1% TTC Solution 20 ml 8588
  100 ml 8589

 


 





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