LOWENSTEIN JENSEN MEDIUM

Technical Data #2509a / 2013.06.26

The Lowenstein Jensen Medium is used for the isolation of Mycobacterium sp. Egg supplement enhances growth of a wide variety of mycobacteria and permits niacin test. Malachite green prevents growth of the majority of contaminants.

 


 

FORMULA
in grams per liter purified filtered water

L-Asparagine

3,6

Monopotassium Phosphate

2,4

Magnesium Sulfate

0,24

Magnesium Citrate

0,6

Potato Flour

30,0

Malachite Green

0,4

Glycerol

12,0 ml

Eggs

1 L

pH 7.2 +/- 0,2 à 25º C

This approximate formula may be adjusted and/or enriched to obtain best results.


PRECAUTIONS
This medium is for IN VITRO diagnostic use only.


STORAGE
Store prepared media at 2-8º C protected from direct light. Medium must not be used if there are signs of contamination or dehydration or if the expiry date has passed. Store dehydrated powder in a dry place in tightly-sealed containers at 2-25º C. Do not use dehydrated media if it is caked or if expiry date has passed. The enrichment must be stored at 2-8º C in a dark place. Do not use if expiry date has passed or if it is contaminated.


DIRECTIONS
Suspend 37,2 g in 600 ml of purified water containing 12 ml of Glycerol. Heat with frequent agitation and boil for one minute. Sterilize at 121º C for 15 minutes. Cool to 45-50º C and aseptically add 1 L of sterile eggs. Dispense into sterile culture tubes and coagulate at 85º C for 45 minutes in slant position.


PROCEDURE
Bring prepared medium at room temperature before inoculation. As usual, decontamination, homogenizing, grinding should be done before inoculation.

  1. Inoculate tubes using light inoculum.
  2. Incubate inoculated tubes with loosened caps, at 35ºC in 5-10% CO2
  3. Read weekly for at least 2 months if there is no growth.


QUALITY CONTROL

Expected results after 21 days at 35ºC 5% CO2

Organisms

ATCC

Growth

Mycobacterium tuberculosis H3Ra

25177

+

Mycobacterium kansasii
Group 1

12478

+

Mycobacterium scrofulaceum
Group II

19981

+

Mycobacterium intracellulare
Group III

13950

+

Mycobacterium fortuitum
Group IV

6841

+


LIMITATIONS OF METHOD
This medium is only a part of identification. Other tests may be required.


REFERENCES

  1. Balows, A., Hausler Jr. W., Herrmann, L.K., Isenberg, D.H., and Shadomy, H.J. 1991 Manual of Clinical Microbiology. 5th ed. American Society for Microbiology. Washington, D.C.
  2. N.C.C.L.S. Quality Assurance for Commercially prepared Microbiological Culture Media. Approved Standard. 1990 Document M22-A.
  3. Sommers, H.M., Mc Clatchy, J.K., Morello, J.A. 1983 Laboratory Diagnosis of the Mycobacteriosis. Cumitech 16. American Society for Microbiology. Washington, D.C.,
  4. Vestal, A.L. 1978 Procedures for the Isolation and Identification of Mycobacterium. DHEW Publication No. CDC 79-8230, CDC Atlanta.


CATALOGUE NUMBERS

Dehydrated media 500 g QB-39-2620
     
Glycerol 500 ml 8466
     
Prepared media    
Tubes 20x125 mm 10/pkg 2509
  100/pkg 2509-H

 


 

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