UVM MODIFIED LISTERIA ENRICHMENT BROTH

Technical Data #2572a / 2013.08.31

UVM Modified Listeria Enrichment Broth is used for the selective isolation and enrichment of Listeria monocytogenes. Esculin is utilized by the Listeria sp., nalidixic acid inhibits gram-negative bacteria and acriflavin gram-positive.

 


 

FORMULA
in grams per liter purified filtered water

Casein Peptone

10,0

Beef Extract

5,0

Yeast Extract

5,0

Sodium Chloride

20,0

Esculin

1,0

Sodium Phosphate, dibasic

9,6

Potassium Phosphate, monobasic

1,35

Acriflavin

0,012

Nalidixic Acid

0,02

pH 7,2 +/- 0,2 à 25º C

This approximate formula may be adjusted and/or enriched to obtain best results.


PRECAUTIONS
This medium is for laboratory use only.


STORAGE
Store prepared media at 2-8º C protected from direct light and dehydrated powder, in a dry place in tightly-sealed containers at 2-25º C.


SIGN OF DETERIORATION
Media should not be used if the expiry date has passed. Prepared media should not be used if there are signs of contamination (shrinking, cracking, evaporation or discoloration). Do not use dehydrated media if it is caked.


DIRECTIONS
Suspend 52 g in 1000 ml of purified water. Heat with frequent agitation and boil for one minute. Dispense into culture tubes. Sterilize at 121º C for 15 minutes.


PROCEDURE
Bring prepared medium at room temperature before inoculation.

  1. Directly, inoculate the specimen.
  2. Incubate at 35º C for 24 hours.


QUALITY CONTROL

Expected results after 24 hrs at 35ºC

Organisms

ATCC

Growth

Listeria monocytogenes

15313

+

Staphylococcus aureus

25923

-

Escherichia coli

25922

-


LIMITATIONS OF METHOD
This medium is only a part of identification. Other tests may be required.


REFERENCES

  1. Lennette, E.H., Ballows, A., Hausler, W.J.Jr., and Shadomy, H.J. Manual of Clinical Microbiology. 4th ed. 1985 Washington D.C.: American Society for Microbiology.
  2. Mac Faddin, Jean F. 1985 Media for Isolation-Cultivation-Identification-Maintenance of Medical Bacteria. Vol. I, Baltimore, MD.: Williams & Wilkins.
  3. Mc Clain, D., W.H. Lee 1988 Development of USDA-FSIS method for isolation of Listeria monocytogenes from raw meat and poultry J. Assoc. Off. Anal. Chem. 71:660-664


CATALOGUE NUMBERS

Dehydrated media 500 g QB-39-5305
     
Prepared media    
Tubes, 16x125 mm 10/pkg 2572
Bottles w/cap, 300ml, 75cm 10 X 225ml 5288

 


 

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