F.B. BROTH (FASTIDIOUS BROTH)

Technical Data #2581a / 2013.06.26

FB Broth, a liquid enriched medium, is used for cultivation of fastidious microorganisms even for specimens that may contain small numbers. Various enrichments enhance fastidious aerobes and anaerobes growth. Agarose replace bacteriological agar to decrease oxygen tension and permit a better visualization of growth.

 


 

FORMULA
in grams per liter purified filtered water

Casein/Meat Peptone

10,0

Casein/Yeast Peptone

10,0

Heart Peptone

5,0

Yeast Extract

5,0

Dextrose

7,5

Sodium Chloride

5,0

L-Cystein HCl

0,1

Sodium Carbonate

0,6

Magnesium Sulfate

0,1

Ferrous Sulfate

0,02

Tris (hydroxymethyl) aminomethane)

0,83

Tris (hydroxymethyl) aminomethane HCI

2,86

Agarose

0,75

Hematin

0,015

Pyridoxal

0,006

NAD

0,015

Tween 80

0,5 ml

pH 7,3 +/- 0,2 à 25º C

This approximate formula may be adjusted and/or enriched to obtain best results.


PRECAUTIONS
This medium is for IN VITRO diagnostic use only.


STORAGE
Store prepared media at 2-8º C protected from direct light. Medium must not be used if there are signs of contamination or if the expiry date has passed.


PROCEDURE
Bring prepared medium to room temperature before use.

  1. Inoculate specimens as soon as possible.
  2. Incubate at 35º C with 5% CO2 during 48-72 hours with loosely caps.
  3. Examine tubes daily for visual signs of growth.
  4. Confirme growth by Gram stain and subculture to general purpose plate.


QUALITY CONTROL

Expected results after 48 hrs at 35ºC W 5% CO2

Organisms

ATCC

Growth

Neisseria gonorrheae

43069

+

Neisseria meningitidis

13090

+

Neisseria gonorrheae

clinical strain

+

Haemophilus influenzae

10211

+

Haemophilus haemolyticus

33390

+

Torulopsis glabrata

clinical strain

+

Streptococcus pneumoniae

6305

+

Streptococcus mitis

clinical strain

+

Streptococcus pyogenes

19615

+

Streptococcus agalactiae

12386

+

Staphylococcus epidermidis

12228

+

Bacteroides fragilis

25285

+

Clostridium difficile

9689

+


LIMITATIONS OF METHOD
This medium is only a part of identification. Other tests may be required.


REFERENCES

  1. Balows, A., W.J. Hausler Jr., K.L. Herrmann, H.D. Isenberg, J. Shadomy. 1991 Manual of Clinical Microbiology. 5th ed. American Society for MIcrobiology. Washington, D.C.
  2. Cartwright, C.P., F. Stock, V.J. Gill. 1994 Improved Enrichment Brothfor Cultivation of Fastidious Organisms. J. Clinic. Microbiol. 32:1825-1826.
  3. Sutter, V.L., D.M. Citron, M.A.C. Edelstein, S.M. Finegold 1986 Wadsworth Anaerobic Bacteriology Manual. 4th ed. Star Publishing Co. Belmont. Calif.


CATALOGUE NUMBERS

Prepared media    
Tubes, 16x125 mm, 10 ml 10/pkg 2581

 


 

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