SCHAEDLER BROTH

Technical Data #2615a / 2013.07.21

Schaedler Broth is a general purpose medium for the cultivation of fastidious aerobic and anaerobic microorganisms. Hemin and cystin enhance growth of some anaerobes.

 


 

FORMULA
in grams per liter purified filtered water

Casein Peptone

8,1

Meat Peptone

2,5

Soy Peptone

1,0

Yeast Extract

5,0

Dextrose

5,82

Sodium Chloride

1,7

Potassium Phosphate, dibasic

0,82

TRIS

3,0

Hemin

0,01

L-Cystine

0,4

pH 7,6 +/- 0,2 à 25º C

This approximate formula may be adjusted and/or enriched to obtain best results.

PRECAUTIONS
This medium is for IN VITRO diagnostic use only.


STORAGE
Store prepared media at 2-8º C protected from direct light and dehydrated powder, in a dry place in tightly-sealed containers at 2-25º C.

SIGN OF DETERIORATION
Media should not be used if the expiry date has passed. prepared media should not be used if there are signs of contamination (evaporation or discoloration). Do not use dehydrated media if it is caked.


DIRECTIONS
Suspend 28,4 g in 1000 ml of purified water. Heat with frequent agitation and boil for one minute. Sterilize at 121º C for 15 minutes. Cool to 45-50º C. Mix thouroughly and dispense into sterile culture tubes.


PROCEDURE

Boil medium for 15 minutes and cool to room temperature before use.

  1. Inoculate specimens as soon as possible. Tighten the caps.
  2. Incubate at 35º C for 24-72 hours and then, up to 7 days.
  3. Examine tubes daily for visual signs of growth.


QUALITY CONTROL

Expected results after 24 hrs at 35ºC

Organisms

ATCC

Growth

Bacteroides fragilis

25285

+

Clostridium perfringens

13124

+

Streptococcus pyogenes

19615

+

LIMITATIONS OF METHOD
This is only a part of the identification. Others tests may be required.

REFERENCES

  1. Lennette, E.H., Ballows, A., Hausler, W.J.Jr., and Shadomy, H.J. Manual of Clinical Microbiology. 4th ed. 1985 Washington D.C.: American society for Microbiology.
  2. Mac Faddin, Jean F. 1985 Media for Isolation-Cultivation-Identification-Maintenance of Medical Bacteria. Vol. I, Baltimore, MD.: Williams & Wilkins.


CATALOGUE NUMBERS

Dehydrated media 500 g QB-39-3821
     
Prepared media    
Tubes 16x125 mm, 5 ml 10/pkg 2615
Bottles 8 oz, 150 ml 10/box 8950

 


 

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