KUPFERBERG TRICHOMONAS BROTH, MODIFIED

Technical Data #2616a / 2006.04.13

Kupferberg Trichomonas Broth Modified is a liquid enriched medium used for isolation and cultivation of Trichomonas Spp. The antibiotic is a selective agent to inhibit the mixed normal flora without any effect on parasite’s recovery. The agar decrease oxygen tension.

 


 

FORMULA
in grams per liter purified filtered water

Casein Peptone

10,0

Meat Peptone

10,0

Maltose

2,0

Cystein HCl

1,0

Chloramphenicol

0,1

Methylene Blue

0,003

Agar

1,0

pH 6,0 +/- 0,2 à 25º C

This approximate formula may be adjusted and/or enriched to obtain best results.


PRECAUTIONS
This medium is for IN VITRO diagnostic use only.


STORAGE
Store prepared media at 2-8° C protected from direct light and dehydrated powder, in a dry place in tightly-sealed containers at 2-25° C.


SIGN OF DETERIORATION
Media should not be used if the expiry date has passed. Prepared media should not be used if there are signs of contamination (evaporation or discoloration). Do not use dehydrated media if it is caked.


DIRECTIONS
Suspend 24,1 g of dehydrated medium in 950 ml of filtered purified water. Heat with frequent agitation and boil for one minute. Sterilize at 121° C for 15 minutes. Cool to 45-50° C and add 50 ml Bovine Serum (#4956). Mix thouroughly and dispense into sterile culture tubes.


PROCEDURE
Bring prepared medium at room temperature before inoculation.

  1. Inoculate broth with clinical specimens.
  2. Incubate at 35° C for up to 7 days before discard. Examine daily under low power of microscope for the presence of typical Trichomonas.


QUALITY CONTROL

Expected results after 24 hrs at 35ºC

Organisms

ATCC

Growth

Trichomonas vaginalis

clinical

+

Staphylococcus aureus

25923

- or partial

Candida albicans

10231

- or partial


LIMITATIONS OF METHOD
This medium is only a part of the identification. Other tests may be required.


REFERENCES

  1. Kupferberg, A.B. 1955 Trichomonas vaginalis: Nutritional Requirements and Diagnostic Procedures. International Rec. Med. And Gen. Practive Clinics, 168:709-717.
  2. Lennette, E.H., Ballows, A., Hausler, W.J.Jr., and Shadomy, H.J. Manual of Clinical Microbiology 4th, ed. 1985 Washington, D.C.: American Society for Microbiology.
  3. Mac Faddin, Jean F. Media for Isolation-Cultivation-Identification-Maintenance of Medical Bacteria. Vol. 1 1985 Baltimore, MD. Williams & Wilkins.


CATALOGUE NUMBERS

Dehydrated media 500 g QB-39-4851
     
Prepared media    
Tubes 16x125 mm, 10 ml 10/pkg 2616
Tubes 16x125 mm, 10 ml 100/box 2616-H

 


 

2325, Dandurand, Suite 300 Phone Fax Internet
Montréal (Quebec) (514) 277-2558 (514) 277-4714 http://www.quelab.com
Canada H2G 1Z9 1 (800) 579-0998 1 (800) 579-8177 info@quelab.com