Technical Data #2638a / 2013.06.30
Phenylalanine Agar is a solid medium used to differentiate Proteus sp. and Providencia sp. from ohter Enterobacteriaceae by their ability to deaminate phenylalanine to phenylpyruvic acid by enzymatic activity. A positive result gives a characteristic dark green color when ferric chloride reagent is added to a pure culture growth.
in grams per liter purified filtered water
|Potassium Phosphate, dibasic||
pH 7,3 +/- 0,2 à 25º C
This approximate formula may be adjusted and/or enriched to obtain best results.
This medium is for IN VITRO diagnostic use only.
Store prepared media at 2-8º C protected from direct light and dehydrated powder, in a dry place, in tightly-sealed containers at 2-25º C.
SIGN OF DETERIORATION
Media should not be used if the expiry date has passed. Prepared media should not be used if there are signs of contamination or deterioration (shrinking, cracking, evaporation or discoloration). Do not use dehydrated media if it is caked.
Suspend 23 g of dehydrated media in 1000 ml of purified filtered water. Heat with frequent agitation and boil for one minute. Sterilize at 121º C for 15 minutes. Cool to 45-50º C. Mix gently and dispense into sterile culture tubes.
Prior to inoculate, the prepared media should be brought to room temperature.
Results after 24 hrs at 35ºC
LIMITATIONS OF METHOD
This medium is only a part of the identification. Other tests may be required.
|Dehydrated media||500 g||QB-39-3525|
|Tubes, 16x125 mm||10/pkg||2638|
|Tubes, 13x100 mm||10/pkg||2742|
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