PEPTONE WATER

Technical Data #2726a / 2013.07.01

Peptone Water is used for the detection of indole and carbohydrate fermentation studies. Addition of carbohydrate and phenol red as indicator, may produce a yellow color for positive results.

 


 

FORMULA
in grams per liter purified filtered water

Casein Peptone

20,0

Sodium Chloride

5,0

pH 7,2 +/- 0,2 à 25º C

This approximate formula may be adjusted and/or enriched to obtain best results.


PRECAUTIONS
This medium is for IN VITRO diagnostic use only.


STORAGE
Store prepared media at 2-8º C protected from direct light and dehydrated powder, in a dry place in tightly-sealed containers at 2-25º C.


SIGN OF DETERIORATION
Media should not be used if the expiry date has passed. Prepared media should not be used if there are signs of contamination (shrinking, cracking, evaporation or discoloration). Do not use dehydrated media if it is caked.


DIRECTIONS
Suspend 25 g in 1000 ml of purified water. Heat with frequent agitation and boil for one minute. Dispense into culture tubes. Sterilize at 121º C for 15 minutes.


PROCEDURE
Bring prepared medium at room temperature before inoculation.

  1. Inoculate with 3-4 similar colonies from an overnight growth.
  2. Incubate at 35º C for 24 hours.
  3. Add a few drops of Kovac's Reagent (#8552).


QUALITY CONTROL

Expected results after 24 hrs at 35ºC

Organisms

ATCC

Growth

Indole

Salmonella typhimurium

14028

+

-

Escherichia coli

25922

+

+

Enterobacter aerogenes

13048

+

-


LIMITATIONS OF METHOD
This medium is only a part of the identification. Other tests may be required.


REFERENCES

  1. Finegold, S.M., W.J. Martin 1982 Bailey and Scott's Diagnostic Microbiology 6th ed. St. Louis. The C.V. Mosby Company
  2. Lennette, E.H., Ballows, A., Hausler, W.J.Jr., and Shadomy, H.J. Manual of Clinical Microbiology. 4th ed. 1985 Washington D.C.: American society for Microbiology.
  3. Mac Faddin, Jean F. 1985 Media for Isolation-Cultivation-Identification-Maintenance of Medical Bacteria. Vol. I, Baltimore, MD.: Williams & Wilkins.


CATALOGUE NUMBERS

Dehydrated media 500 g QB-39-2107
     
Prepared media    
Tube 16x125mm, 5 ml 10/pkg 2726

 


 

 

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