COOKED MEAT MEDIUM WITH FLUID THIOGLYCOLLATE

Technical Data #2762a / 2013.07.01

A liquid medium used for cultivating anaerobic microorganisms. The solid meat particles has the capacity to initiate growth of bacteria from the moment of inoculation and maintain viability of cultures over long periods of time. Fluid thioglycollate develop a faster growth of anaerobes. Sodium thioglycollate is used as reducing agent, agar stop oxygen diffusion through the medium and resazurin is the oxidation-reduction indicator.

 


 

FORMULA
in grams per liter purified filtered water

Beef Heart

100,0

Proteose Peptone

20,0

Dextrose

7,5

Sodium Chloride

7,5

Casein Peptone

15,0

Yeast Extract

5,0

L-Cystine

0,5

Sodium Thioglycollate

0,5

Resazurin

0,001

Agar

0,75

pH 7,2 +/- 0,2 à 25º C

This approximate formula may be adjusted and/or enriched to obtain best results.


PRECAUTIONS
This medium is for IN VITRO diagnostic use only.


STORAGE
Store prepared media at 2-25º C protected from direct light. Medium must not be used if there are signs of contamination or if the expiry date has passed. Store dehydrate powder in a dry place in tightly-sealed containers at 2-25º C. Do not use dehydrated media if expiry date has passed.


DIRECTIONS
Place 1,25 grams of dehydrated medium into a culture tube. Add 10 ml of Fluid Thioglycollate Medium. Mix well and allow to stand 10 minutes. Sterilize at 121º C for 15 minutes. Fluid Thioglycollate Medium is prepared by suspending 29,8 grams of Dehydrated Medium in 1000 ml of purified filtered water. After mixing, heat with frequent agitation and boil for one minute.


PROCEDURE
Liquid media for anaerobic incubation should be reduced prior to inoculation under anaerobic conditions for 18-24 h, cap loosened, or they may be reduced immediately prior to use by boiling for 10 minutes, cap loosened, and cooling at room temperature.
Anaerobic Culture (general): Inoculated Cooked Meat Medium with Fluid Thioglycollate are incubated with loosened caps at 35ºC in an anaerobic environment. Examine cultures for up 10 days. Observe for gas production and digestion of meat particles.
Aerobic culture: Incubate cultures at 35º C. Observe daily for 5-10 days.

QUALITY CONTROL
Expected results at 35ºC

Organisms

ATCC

Growth

C. perfringens

13124

+

B. fragilis

25285

+

B. vulgatus

14464

+

C. difficile

9689

+

C. novyi A

7659

+

B. levii

29147

+


LIMITATIONS OF METHOD
Each growth should be confirmed aerobically and anaerobically to recover bacteria.


REFERENCES

  1. Mac Faddin, J.F. 1985 Media for isolation-cultivation-identification-maintenance of medical bacteria, vol I. Williams and Williams, Baltimore
  2. Lennette, E.H. et al. 1985 Manual of Clinical Microbiology 4th ed American Society of Microbiology, Washington
  3. Dowell, V.R., G.L. Lombard, F.S. Thompson, A.Y. Armfield. 1977 Media for isolation, characterization and identification of obligately anaerobic bacteria. CDC laboratory manual Center for Disease Control, Atlanta.


CATALOGUE NUMBERS

Prepared media    
Tubes 16x125 mm 10/pkg 2762
  100/bx 2762-H

 


 

 

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