Technical Data #2815a / 2013.07.02
Selenite Broth is a selective enrichment medium used for cultivation of Salmonella and some Shigella sp. Specimens from feces, urine, water and foods may be inoculated even if quantity is very low. Sodium selenite inhibits gram-positive cocci as enterococci and gram-negative bacteria as coliforms.
in grams per liter purified filtered water
pH 7.0 +/- 0,2 à 25º C
This approximate formula may be adjusted and/or enriched to obtain best results.
This medium is for IN VITRO diagnostic use only.
Store prepared media at 2-8º C protected from direct light and dehydrated powder, in a dry place, in tightly-sealed containers at 2-25º C.
SIGN OF DETERIORATION
Media should not be used if the expiry date has passed. Prepared media should not be used if there are signs of contamination or deterioration (shrinking, cracking, evaporation or discoloration, or if red color appears). Do not use dehydrated media if it is caked or if an orange color is developped after container is opened.
Suspend 23 g of dehydrated media in 1000 ml of purified filtered water. Heat with frequent agitation and boil for one minute. DO NOT STERILIZE. Cool to 45-50º C. Mix gently and dispense into sterile culture tubes.
Prior to inoculate, the prepared media should be brought to room temperature.
Results after 24 hrs at 35ºC
+ or partial inhibition
- or partial
LIMITATIONS OF METHOD
Do not incubate broth longer than 24 hours. This medium is only a part of the identification. Other tests may be required.
|Dehydrated media||500 g||QB-39-3810|
|Tubes, 16x125 mm||10/pkg||2815|
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