Technical Data #2833a / 2013.07.02
Rapid Hippurate Test Broth are used to differentiate bovine -haemolytic Group B Streptococcus from human -haemolytic Group B Streptococcus on the basis of their ability to hydrolyze sodium hippurate by enzymatic activity producing benzoic acid as final product. Addition of Ninhydrine is necessary to confirm a positive reaction.
in grams per liter purified filtered water
This approximate formula may be adjusted and/or enriched to obtain best results.
This medium is for IN VITRO diagnostic use only.
Store prepared media at -10º C protected from direct light.
SIGN OF DETERIORATION
Media should not be used if the expiry date has passed. Prepared media should not be used if there are signs of contamination or deterioration (evaporation or discoloration). Do not use powder if it is caked.
Suspend 10 g in 1000 ml of purified filtered water.Heat gently to dissolve. Dispense into culture tubes. Sterilize at 121º C for 15 minutes. Cool to room temperature.
Prior to inoculate, the prepared media should be brought to room temperature.
Positive Reaction: purple color within 15 minutes.
Negative Reaction: no color change.
Results after 4 hrs at 35ºC
LIMITATIONS OF METHOD
Strain of Streptococcus should be confirmed before inoculation of medium. This medium is only a part of the identification. Other tests may be required.
|Sodium Hippurate, powder||25 g||QB-63-9380|
|Ninhydrin 3.5%, solution||30 ml||8556|
|Tubes, 13x100 mm||10/pkg||2833|
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