RAPID HIPPURATE TEST BROTH

Technical Data #2833a / 2013.07.02

Rapid Hippurate Test Broth are used to differentiate bovine -haemolytic Group B Streptococcus from human -haemolytic Group B Streptococcus on the basis of their ability to hydrolyze sodium hippurate by enzymatic activity producing benzoic acid as final product. Addition of Ninhydrine is necessary to confirm a positive reaction.

 


 

FORMULA
in grams per liter purified filtered water

Sodium Hippurate

10,0

This approximate formula may be adjusted and/or enriched to obtain best results.


PRECAUTIONS
This medium is for IN VITRO diagnostic use only.


STORAGE
Store prepared media at -10º C protected from direct light.


SIGN OF DETERIORATION
Media should not be used if the expiry date has passed. Prepared media should not be used if there are signs of contamination or deterioration (evaporation or discoloration). Do not use powder if it is caked.


DIRECTIONS
Suspend 10 g in 1000 ml of purified filtered water.Heat gently to dissolve. Dispense into culture tubes. Sterilize at 121º C for 15 minutes. Cool to room temperature.


PROCEDURE
Prior to inoculate, the prepared media should be brought to room temperature.

  1. Inoculate tubes with a heavily suspension of pure culture from identified Streptococcus growth.
  2. Incubate at 35º C for at least 2-4 hours, depending on inoculum.
  3. Add 4 drops of Ninhydrin Reagent, mix thouroughly and reincubate at 35º C for 10-15 minutes.

Positive Reaction: purple color within 15 minutes.
Negative Reaction: no color change.


QUALITY CONTROL

Results after 4 hrs at 35ºC

Organisms

ATCC

Reaction

Streptococcus agalactiae

12386

+

Streptococcus pyogenes

19615

-


LIMITATIONS OF METHOD
Strain of Streptococcus should be confirmed before inoculation of medium. This medium is only a part of the identification. Other tests may be required.


REFERENCES

  1. Edberg, S.C., S. Samuels 1976 Rapid colorimetric test for determination of hippurate hydrolysis of group B Streptococcus J. Clin. Microbiol. 3(1):49
  2. Hwang, M., G.M. Ederer 1975 Rapid hippurate hydrolysis method for presumptive identification of group B streptococci. J. Clin. Microbiol. 1(1):114.
  3. Lennette, E.H., Ballows, A., Hausler, W.J.Jr., and Shadomy, H.J. Manual of Clinical Microbiology. 4th ed. 1985 Washington D.C.: American society for Microbiology.
  4. Mac Faddin, J.F. 1985 Media for isolation-cultivation-identification-maintenance of medical bacteria Williams and Wilkins, Baltimore


CATALOGUE NUMBERS

Sodium Hippurate, powder 25 g QB-63-9380
     
Ninhydrin 3.5%, solution 30 ml 8556
     
Prepared media    
Tubes, 13x100 mm 10/pkg 2833

 


 

 

2325, Dandurand, Suite 300 Phone Fax Internet
Montréal (Quebec) (514) 277-2558 (514) 277-4714 http://www.quelab.com
Canada H2G 1Z9 1 (800) 579-0998 1 (800) 579-8177 info@quelab.com