INFUSION BROTH WITH SODIUM CHLORIDE 6,5%

Technical Data #2877a / 2013.07.06

Infusion Broth with 6,5% Sodium Chloride is a liquid medium used to differentiate enterococci from other streptococci by addition of 65 grams per liter of sodium chloride. Sodium chloride reacts as selective agents because mostly of Enterococci sp. may grow in this selective broth.

 


 

FORMULA
in grams per liter purified filtered water

Beef Heart Infusion

2,0

Casein Peptone

10,0

Meat Peptone

5,0

Yeast Extract

3,0

Sodium Chloride

65,0

pH 7,3 +/- 0,2 à 25º C

This approximate formula may be adjusted and/or enriched to obtain best results.


PRECAUTIONS
This medium is for IN VITRO diagnostic use only.


STORAGE
Store prepared media at 2-8º C protected from direct light and dehydrated powder, in a dry place in tightly-sealed containers at 2-25º C.


SIGN OF DETERIORATION
Media should not be used if the expiry date has passed. Prepared media should not be used if there are signs of contamination (evaporation or discoloration). Do not use dehydrated media if it is caked.


DIRECTIONS
Suspend 20 g in 1000 ml of purified filtered water. Add 60 grams of sodium chloride, heat with frequent agitation and boil for one minute. Mix thouroughly and dispense into culture tubes. Sterilize at 121º C for 15 minutes.


PROCEDURE
Bring prepared medium at room temperature before inoculation.

  1. Inoculate with 3-5 similar colonies from an overnight growth.
  2. Incubate at 35º C for 48-72 hours.
  3. Examine daily for growth.


QUALITY CONTROL

Expected results after 24 hrs at 35ºC

Organisms

ATCC

Growth

Enterococcus faecalis

29212

+

Streptococcus pyogenes

19615

-

Streptococcus bovis

9809

-


LIMITATIONS OF METHOD
Negative result may be confirmed only after 3 days of incubation. This is only a part of the identification. Others tests may be required.


REFERENCES

  1. Facklam, R.R., 1973 Comparaison of several laboratory media for presumptive identification of enterococci and group D streptococci. Appl. Microbiol. 26:138.
  2. Lennette, E.H., Ballows, A., Hausler, W.J.Jr., and Shadomy, H.J. Manual of Clinical Microbiology. 4th ed. 1985 Washington D.C.: American society for Microbiology.
  3. Mac Faddin, Jean F. 1985 Media for Isolation-Cultivation-Identification-Maintenance of Medical Bacteria. Vol. I, Baltimore, MD.: Williams & Wilkins.


CATALOGUE NUMBERS

Dehydrated media 500 g QB-39-1929
Sodium Chloride 500 g QB-39-4109
     
Prepared media    
Tubes 13x100 mm, 2 ml 10/pkg 2877
Tubes 13x100 mm, 2 ml 100/pkg 2877-H

 


 

 

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