Technical Data #2877a / 2013.07.06
Infusion Broth with 6,5% Sodium Chloride is a liquid medium used to differentiate enterococci from other streptococci by addition of 65 grams per liter of sodium chloride. Sodium chloride reacts as selective agents because mostly of Enterococci sp. may grow in this selective broth.
in grams per liter purified filtered water
|Beef Heart Infusion||
pH 7,3 +/- 0,2 à 25º C
This approximate formula may be adjusted and/or enriched to obtain best results.
This medium is for IN VITRO diagnostic use only.
Store prepared media at 2-8º C protected from direct light and dehydrated powder, in a dry place in tightly-sealed containers at 2-25º C.
SIGN OF DETERIORATION
Media should not be used if the expiry date has passed. Prepared media should not be used if there are signs of contamination (evaporation or discoloration). Do not use dehydrated media if it is caked.
Suspend 20 g in 1000 ml of purified filtered water. Add 60 grams of sodium chloride, heat with frequent agitation and boil for one minute. Mix thouroughly and dispense into culture tubes. Sterilize at 121º C for 15 minutes.
Bring prepared medium at room temperature before inoculation.
Expected results after 24 hrs at 35ºC
LIMITATIONS OF METHOD
Negative result may be confirmed only after 3 days of incubation. This is only a part of the identification. Others tests may be required.
|Dehydrated media||500 g||QB-39-1929|
|Sodium Chloride||500 g||QB-39-4109|
|Tubes 13x100 mm, 2 ml||10/pkg||2877|
|Tubes 13x100 mm, 2 ml||100/pkg||2877-H|
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