Technical Data #2899a / 2013.07.09
Litmus Milk Broth is a liquid medium used to aid in species differentiation primarily from the genus Clostridium, of Streptococcus bovis from Streptococcus equinus, maintenance of lactic acid bacteria associated with dairy products and detection of Bacillus cereus in foods.
FORMULA
in grams per liter purified filtered water
Skim Milk |
100,0 |
Litmus |
0,75 |
pH 6,8 +/- 0,2 à 25º C |
This approximate formula may be adjusted and/or enriched to obtain best results.
PRECAUTIONS
This medium is for IN VITRO diagnostic use only.
STORAGE
Store prepared media at 2-8º C protected from direct light and dehydrated powder, in a dry place in tightly-sealed containers at 2-25º C.
SIGN OF DETERIORATION
Media should not be used if the expiry date has passed. Prepared media should not be used if there are signs of contamination (evaporation or discoloration). Do not use dehydrated media if it is caked.
DIRECTIONS
Suspend 100,8 g in 1000 ml of purified water. Heat gently into solution. Mix thouroughly and dispense into culture tubes. Sterilize at 118º C for 15 minutes. AVOID OVERHEATING.
PROCEDURE
Before inoculation, boil for 2 minutes and bring prepared medium at room temperature.
QUALITY CONTROL
Expected results after 7 days at 35ºC
Organisms |
ATCC |
Growth |
Clostridium perfringens |
13124 |
Acid, w/ gas |
Pseudomonas aeruginosa |
27853 |
Peptonized |
Clostridium sporogenes |
11437 |
Acid, clot, proteolysis |
Enterococcus faecalis |
29212 |
Acid, clot |
LIMITATIONS OF METHOD
During storage, medium may show a layered coloration due to the oxidation of the top of the liquid, boiling during 2 minutes will drive off oxygen. This is only a part of the identification. Others tests may be required.
REFERENCES
CATALOGUE NUMBERS
Dehydrated media | 500 g | QB-39-3409 |
Prepared media | ||
Tubes 16x125 mm, 5 ml | 10/pkg | 2899 |
2325, Dandurand, Suite 300 | Phone | Fax | Internet |
Montréal (Quebec) | (514) 277-2558 | (514) 277-4714 | http://www.quelab.com |
Canada H2G 1Z9 | 1 (800) 579-0998 | 1 (800) 579-8177 | info@quelab.com |