TRYPTOSE BROTH W/THIAMINE

Technical Data #2936a / 2013.04.12

Tryptose broth w/Thiamine is a liquid general purpose medium for the cultivation of pathogenic organisms without enrichment, for streptococci, pneumococci, meningococci and other fastidious aerobic microorganisms.

FORMULA
in grams per liter purified filtered water

Casein/Yeast Peptone	20,0
Dextrose	1,0
Sodium Chloride	5,0
Thiamine HCl	0,005

pH 7,2 +/- 0,2 à 25º C

This approximate formula may be adjusted and/or enriched to obtain best results.

PRECAUTIONS
This medium is for laboratory use only.

STORAGE
Store prepared media at 2-8º C protected from direct light and dehydrated powder, in a dry place in tightly-sealed containers at 2-25º C.

SIGN OF DETERIORATION
Media should not be used if the expiry date has passed. Prepared media should not be used if there are signs of contamination (discoloration). Do not use dehydrated media if it is caked.

DIRECTIONS
Suspend 26 g in 1000 ml of purified water. Heat with frequent agitation and boil for one minute to dissolve completely. Distribute in tubes or bottles and sterilize in the autoclave for 15 minutes at 121º C (15 lbs pressure).

PROCEDURE
Bring prepared medium to room temperature before inoculation. For best results the medium should be freshly prepared. If not used the same day as sterilized, heat in boiling water to remove absorbed oxygen and cool quickly without agitation just prior to inoculation.

1. Inoculate according to previous standardized procedures.
2. Incubate at 35º C for 24-48 hours with loosened caps.
3. Examine for growth.

QUALITY CONTROL
Expected results after 24 hrs at 35ºC

Organisms	ATCC	GROWTH
Streptococcus pneumoniae	6305	+
Streptococcus pyogenes	19615	+
Neisseria meningitidis	13090	+
Brucella abortus	4315	+

LIMITATIONS OF METHOD
This medium is only a part of identification. Other tests may be required.

REFERENCES

1. Lennette, E.H., Ballows, A., Hausler, W.J.Jr., and Shadomy, H.J. Manual of Clinical Microbiology. 4th ed. 1985 Washington D.C.: American society for Microbiology.
2. Mac Faddin, Jean F. 1985 Media for Isolation-Cultivation Identification-Maintenance of Medical Bacteria. Vol. I, Williams & Wilkins, Baltimore, Md.
3. Huddleson. 1943. Brucellose in man and animals, rev.ed. The Commonwealth Fund, New York, NY
4. U.S. Food and Drugs Administration. 1995. Bacteriological analytical manual, 8th ed. AOAC International, Gaithersburg, Md.

CATALOGUE NUMBERS

Dehydrated media	500 grams	QB-39-5209
Prepared media
Tubes 16x125 mm, 5 ml	10/pkg	2936
Tubes 16x125 mm, 10 ml	10/pkg	2930