TRYPTOSE BROTH

Technical Data #2983a / 2013.04.12

Tryptose broth is a liquid medium for the isolation, cultivation and differentiation of Brucella and other fastidious microorganisms. Prepared without extract or infusion of meat it is recommended for the cultivation of discriminating pathogenic as well as saprophytic bacteria.

 


 

FORMULA
in grams per liter purified filtered water

Casein/Yeast Peptone

20,0

Dextrose

1,0

Sodium Chloride

5,0

pH 7,2 +/- 0,2 à 25º C

This approximate formula may be adjusted and/or enriched to obtain best results.


PRECAUTIONS
This medium is for laboratory use only.


STORAGE
Store prepared media at 2-8º C protected from direct light and dehydrated powder, in a dry place in tightly-sealed containers at 2-25º C.


SIGN OF DETERIORATION
Media should not be used if the expiry date has passed. Prepared media should not be used if there are signs of contamination (discoloration). Do not use dehydrated media if it is caked.


DIRECTIONS
Suspend 26 g in 1000 ml of purified water. Heat with frequent agitation and boil for one minute to dissolve completely. Distribute in tubes or bottles and sterilize in the autoclave for 15 minutes at 121º C (15 lbs pressure).


PROCEDURE
Bring prepared medium to room temperature before inoculation. For best results the medium should be freshly prepared. If not used the same day as sterilized, heat in boiling water to remove absorbed oxygen and cool quickly without agitation just prior to inoculation.

  1. Inoculate according to previous standardized procedures.
  2. Incubate at 35º C for 24-48 hours with loosened caps.
  3. Examine for growth.


QUALITY CONTROL

Expected results after 24 hrs at 35ºC

Organisms

ATCC

GROWTH

Brucella abortus

11192

+

Brucella melitensis

4309

+

Brucella suis

9843

+


LIMITATIONS OF METHOD
This medium is only a part of identification. Other tests may be required.


REFERENCES

  1. Huddleson. 1943. Brucellosis in man and animals, rev. ed. The commonwealth Fund, New York, NY.
  2. Huddleson. 1939. Brucellosis in man and animals,. Oxford University Press, Oxford, England
  3. Mac Faddin, Jean F. 1985 Media for Isolation-Cultivation Identification-Maintenance of Medical Bacteria. Vol. I, Williams & Wilkins, Baltimore, Md.
  4. Standard Methods for the Examination of Dairy Products, 1948 9th Ed.: 165
  5. Atlas.1995. Handbook of microbiology media for the examination of food. CRC Press, Boca Raton, Flo.
  6. Ruoff, Whiley and Beighton. 1999. In Murray, Baron, Pfaller, Tenover and Yolken (ed), manual of clinical microbiology, 7th ed. American Society for Microbiology, Washington, DC.


CATALOGUE NUMBERS

Dehydrated media 500 grams QB-39-5020
     
Prepared media    
Tubes 16x125 mm, 5 ml 10/pkg 2983
Tubes 16x125 mm, 10 ml 10/pkg 2922

 


 

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