Technical Data #2983a / 2013.04.12
Tryptose broth is a liquid medium for the isolation, cultivation and differentiation of Brucella and other fastidious microorganisms. Prepared without extract or infusion of meat it is recommended for the cultivation of discriminating pathogenic as well as saprophytic bacteria.
in grams per liter purified filtered water
pH 7,2 +/- 0,2 à 25º C
This approximate formula may be adjusted and/or enriched to obtain best results.
This medium is for laboratory use only.
Store prepared media at 2-8º C protected from direct light and dehydrated powder, in a dry place in tightly-sealed containers at 2-25º C.
SIGN OF DETERIORATION
Media should not be used if the expiry date has passed. Prepared media should not be used if there are signs of contamination (discoloration). Do not use dehydrated media if it is caked.
Suspend 26 g in 1000 ml of purified water. Heat with frequent agitation and boil for one minute to dissolve completely. Distribute in tubes or bottles and sterilize in the autoclave for 15 minutes at 121º C (15 lbs pressure).
Bring prepared medium to room temperature before inoculation. For best results the medium should be freshly prepared. If not used the same day as sterilized, heat in boiling water to remove absorbed oxygen and cool quickly without agitation just prior to inoculation.
Expected results after 24 hrs at 35ºC
LIMITATIONS OF METHOD
This medium is only a part of identification. Other tests may be required.
|Dehydrated media||500 grams||QB-39-5020|
|Tubes 16x125 mm, 5 ml||10/pkg||2983|
|Tubes 16x125 mm, 10 ml||10/pkg||2922|
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