m-GREEN YEAST & MOLD BROTH

Technical Data #39-1912a / 2013.07.21

m-Green Yeast & Mold Broth is used for the detection of fungi in the routine analysis of beverages. Several nutrients factors permit luxuriant fungal growth, acidic pH inhibits bacteria and bromocresol green is the color indicator.

 


 

FORMULA
in grams per liter purified filtered water

Casein Peptone

5,0

Meat Peptone

5,0

Yeast Extract

9,0

Dextrose

50,0

Magnesium Sulfate

2,1

Potassium Phosphate

2,0

Diastase

0,05

Thiamine

0,05

Bromcresol Green

0,026

pH 7,2 +/- 0,2 à 25º C

This approximate formula may be adjusted and/or enriched to obtain best results.


PRECAUTIONS
This medium is for IN VITRO diagnostic use only.


STORAGE
Store prepared media at 2-8º C protected from direct light and dehydrated powder, in a dry place in tightly-sealed containers at 2-25º C.


SIGN OF DETERIORATION
Media should not be used if the expiry date has passed. Prepared media should not be used if there are signs of contamination (shrinking, cracking, evaporation or discoloration) Do not use dehydrated media if it is caked.


DIRECTIONS
Suspend 73 g in 1000 ml of purified water. Heat slightly with frequent agitation to dissolve. Dispense into culture tubes. Sterilize at 118º C for 15 minutes.


PROCEDURE
Bring prepared medium at room temperature before inoculation.

  1. Saturate a sterile membrane filter pad with 2 ml of m-Green Yeast and Fungi Broth.
  2. Roll the membrane filter through which the sample has been filtered onto the saturated pad; avoid the trapping of air bubbles between the filter and the pad.
  3. Incubate at 30-35º C for 48 hours.


QUALITY CONTROL

Expected results after 48 hrs at 35ºC

Organisms

ATCC

Growth

Saccharomyces cerevisiae

9763

+

Aspergillus niger

16404

+


LIMITATIONS OF METHOD
This medium is only a part of the identification. Other tests may be required.


REFERENCES

  1. Lennette, E.H., Ballows, A., Hausler, W.J.Jr., and Shadomy, H.J. Manual of Clinical Microbiology. 4th ed. 1985 Washington D.C.:American society for Microbiology.
  2. Mac Faddin, Jean F., Media for Isolation-Cultivation-Identification-Maintenance of Medical Bacteria Vol.1 1985 Baltimore, MD. Williams & Wilkins.
  3. NCCLS 1990 Quality Assurance for Commercially Prepared Microbiological Culture Mediaicrobiology. Approved Standard Document M22-A Vol.10, No.14


CATALOGUE NUMBERS

Dehydrated media 500 g QB-39-1912

 


 

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