m-FC BROTH

Technical Data #39-2910a / 98.07.21

m-FC Broth is a selective broth used for the detection of fecal coliforms as an index of water pollution using a membrane filter technique at elevated temperature. Bile salts inhibit gram positive bacteria. With addition of Rosolic Acid, fecal coliforms colonies are blue.

 


 

FORMULA
in grams per liter purified filtered water

Casein Peptone

9,0

Meat Peptone

2,5

Yeast Extract

6,5

Sodium Chloride

5,0

Lactose

12,5

Bile Salts

1,5

Aniline Blue

0,1

pH 7,4 +/- 0,2 à 25º C

This approximate formula may be adjusted and/or enriched to obtain best results.


PRECAUTIONS
This medium is for IN VITRO diagnostic use only.


STORAGE
Store prepared media at 2-8º C protected from direct light and dehydrated powder, in a dry place in tightly-sealed containers at 2-25º C.


SIGN OF DETERIORATION
Media should not be used if the expiry date has passed. Prepared media should not be used if there are signs of contamination (shrinking, cracking, evaporation or discoloration). Do not use dehydrated media if it is caked.


DIRECTIONS
Suspend 37 g in 1000 ml of purified water. Heat with frequent agitation and boil for one minute. Add 10 ml of 1% Rosolic Acid Solution. Dispense into sterile culture tubes. DO NOT AUTOCLAVE.


PROCEDURE
Bring prepared medium at room temperature before inoculation.

  1. Saturate an absorbent pad in tight-fitting plastic dishes with broth.
  2. Transfer the membrane filter used to collect specimen onto the moistened pad. Avoid air bubbles between membrane and pad.
  3. Incubate, by immersion, in a water bath at 44º C for 24 hours.
  4. Examine for growth.


QUALITY CONTROL

Expected results after 24 hrs at 35ºC

Organisms

ATCC

Growth

Color

Escherichia coli

25922

+

blue

Enterobacter aerogenes

13048

+

gray

Salmonella typhimurium

14028

-

 


LIMITATIONS OF METHOD
This medium is only a part of the identification. Other tests may be required.


REFERENCES

  1. Mac Faddin, Jean F. 1985 Media for Isolation-Cultivation-Identification-Maintenance of Medical Bacteria. Vol. I, Baltimore, MD.: Williams & Wilkins.
  2. Standards Methods for the examination of water and wastewater 16th ed. A.P.H.A. 1985 Washington, DC


CATALOGUE NUMBERS

Dehydrated media 500 g QB-39-2910

 


 

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