m-ENDO BROTH

Technical Data #39-2915a / 2013.07.21

m-Endo Broth is a selective broth used for the enumeration of coliforms by membrane filtration. Sodium deoxycholate and lauryl sulfate inhibit gram-positive bacteria. Lactose fermenters react with basic fuchsine and change medium color to red.

 


 

FORMULA
in grams per liter purified filtered water

Casein Peptone

10,0

Meat Peptone

10,0

Yeast Extract

1,5

Sodium Chloride

5,0

Lactose

12,5

Sodium Deoxycholate

0,1

Potassium Phosphate, dibasic

4,375

Potassium Phosphate, monobasic

1,375

Sodium Lauryl Sulfate

0,05

Sodium Sulfite

2,1

Basic Fuchsine

1,05

pH 7,2 +/- 0,2 à 25º C

This approximate formula may be adjusted and/or enriched to obtain best results.


PRECAUTIONS
This medium is for IN VITRO diagnostic use only.


STORAGE
Store prepared media at 2-8º C protected from direct light and dehydrated powder, in a dry place in tightly-sealed containers at 2-25º C.


SIGN OF DETERIORATION
Media should not be used if the expiry date has passed. Prepared media should not be used if there are signs of contamination (shrinking, cracking, evaporation or discoloration). Do not use dehydrated media if it is caked.


DIRECTIONS
Suspend 48 g in 1000 ml of purified water containing 20 ml of ethanol. Heat with frequent agitation and boil for one minute. Dispense into sterile culture tubes. DO NOT AUTOCLAVE.


PROCEDURE
Bring prepared medium at room temperature before inoculation.

  1. Saturate a membrane filter pad with broth and place it in a Petri dish.
  2. Transfer the membrane filter used to collect specimen onto the moistened pad. Avoid air bubbles between membrane and pad.
  3. Incubate at 35º C for 24 hours.
  4. Examine for growth.


QUALITY CONTROL

Expected results after 24 hrs at 35ºC

Organisms

ATCC

Growth

Color

Escherichia coli

25922

+

red/metallic sheen

Enterobacter aerogenes

13048

+

red/metallic sheen

Staphylococcus aureus

25923

- or partial

 


LIMITATIONS OF METHOD
This medium is only a part of the identification. Other tests may be required.


REFERENCES

  1. Mac Faddin, Jean F. 1985 Media for Isolation-Cultivation-Identification-Maintenance of Medical Bacteria. Vol. I, Baltimore, MD.: Williams & Wilkins.
  2. Standards Methods for the examination of water and wastewater 16th ed. A.P.H.A. 1985 Washington, DC


CATALOGUE NUMBERS

Dehydrated media 500 g QB-39-2915

 


 

 

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