MYCOLOGICAL AGAR WITH LOW pH

Technical Data #39-3016a / 2013.08.18

Mycological Agar with low pH is a solid selective medium used for isolation, identification and cultivation of yeasts and molds and saprophytic yeasts and molds in sugars, beverages and foods. It may be used for isolation of aciduric bacteria Peptone and dextrose are the nutrious agents for yeasts and molds recovery.

 


 

FORMULA
in grams per liter purified filtered water

Soy Peptone

10,0

Dextrose

10,0

Agar

15,0

pH 4,8 +/- 0,2 à 25º C

This approximate formula may be adjusted and/or enriched to obtain best results.


PRECAUTIONS
This medium is for laboratory use only.


STORAGE
Store dehydrated powder, in a dry place, in tightly-sealed containers at 2-25° C.


SIGN OF DETERIORATION
Media should not be used if the expiry date has passed. Do not use dehydrated media if it is caked.


DIRECTIONS
Suspend 35 g of dehydrated media into 1000 ml of purified filtered water. Heat with frequent agitation and boil for one minute. Autoclave at 121° C for 15 minutes, cool down to 45-50° C and dispense into sterile Petri dishes. Alternatively, dispense into culture tubes and sterilize at 121° C for 15 minutes. Cool in a slanted position. DO NOT OVERHEAT.


PROCEDURE
Prior to inoculate, the prepared media should be brought to room temperature.

  1. Inoculate prepared media with specimens as soon as possible and streak to obtain isolated colonies. Inoculate in duplicate.
  2. Incubate one set of prepared media at 25° C for up to 4-6 weeks and the other set at 35° C for up to 7 days.
  3. Examine frequently for growth.


QUALITY CONTROL

Results after 7 days at 25ºC

Organisms

ATCC

Growth

Candida albicans

10231

+

Candida albicans

60193

+

Aspergillus niger

16404

+

Saccharomyces cerevisiae

9763

+


LIMITATIONS OF METHOD
This medium is only a part of the identification. Other tests may be required.


REFERENCES

  1. Lennette, E.H., Ballows, A., Hausler, W.J.Jr., and Shadomy, H.J. Manual of Clinical Microbiology. 4th ed. 1985 Washington D.C.: American Society for Microbiology.
  2. Mac Faddin, Jean F., Media for Isolation-Cultivation-Identification-Maintenance of Medical Bacteria Vol.1 1985 Baltimore, MD. Williams & Wilkins.
  3. Isenberg, ed 1992. Clinical microbiology procedures handbookAmerican Society for Microbiology, Washington. D.C.


CATALOGUE NUMBERS

Dehydrated powder 500 g QB-39-3016

 


 

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