SCHAEDLER AGAR

Technical Data #39-3835a / 2013.07.21

Schadler Agar may be used for cultivation and isolation of fastidious aerobes and of a large quantity of obligate and facultative anaerobes when Vitamin K and sheep blood are added into the medium. Hemin and cystine enhance the growth of some anaerobes.

 


 

FORMULA
in grams per liter purified filtered water

Casein Peptone

8,2

Meat Peptone

2,5

Soy Peptone

1,0

Yeast Extract

5,0

Dextrose

5,8

Sodium Chloride

1,7

Dipotassium Phosphate

0,8

L-Cystine

0,4

Hemin

0,01

Tris (Hydroxymethylaminomethane)

3,0

Agar

13,5

pH 7,6 +/- 0,2 à 25º C

This approximate formula may be adjusted and/or enriched to obtain best results.


PRECAUTIONS
This medium is for IN VITRO diagnostic use only.


STORAGE
Store prepared media at 2-8º C protected from direct light and dehydrated powder, in a dry place, in tightly-sealed containers at 2-25º C.


SIGN OF DETERIORATION
Media should not be used if the expiry date has passed. Prepared media should not be used if there are signs of contamination or deterioration (shrinking, cracking, evaporation or discoloration). Do not use dehydrated media if it is caked.


DIRECTIONS
Suspend 41,9 g of dehydrated media in 950 ml of purified filtered water. Heat with frequent agitation and boil for one minute. Sterilize at 121º C for 15 minutes. Cool to 45-50º C and aseptically, add 50 ml of Sheep Blood (4245), 1 ml of a sterile solution of Vitamin K. Mix gently and dispense into sterile Petri dishes.


PROCEDURE
Prior to inoculate, the prepared media should be brought to room temperature.

  1. Suitable anaerobes recovery is depending of collection and transportation of specimens. Specimens should be transferred into Anaerobes Pre-Reduced Transport Medium (2017) immediately after collection and brought to the laboratory quickly.
  2. Directly, inoculate plate as soon as possible with a large amount of inoculum, streak for isolation.
  3. Incubate at 35º C under anaerobic conditions for 2-4 days.
  4. Examine for growth.


QUALITY CONTROL

Results after 48 hrs at 35ºC anaerobic atmosphere

Organisms

ATCC

Growth

Bacteroides fragilis

25285

+

Clostridium perfringens

13124

+ hemolysis

Fusobacterium nucleatum

25586

+

Bacteroides levii

29147

+

Peptostreptococcus anaerobius

27337

+


LIMITATIONS OF METHOD
This medium is only a part of the identification. Other tests may be required.


REFERENCES

  1. Lennette, E.H., Ballows, A., Hausler, W.J.Jr., and Shadomy, H.J. Manual of Clinical Microbiology. 4th ed. 1985 Washington D.C.: American Society for Microbiology.
  2. Mac Faddin, J.F. 1985 Media for Isolation-Cultivation-Identification-Maintenance of Medical Bacteria Vol. I Baltimore, MD.: Williams & Wilkins.
  3. Mata, L.J., C. Carrillo, E. Villatoro. 1969 Fecal microflora in healthy persons in the preindustrial region. Appl. Microbiol. 17:596.
  4. Sutter, V.L., D.M. Citron, M.A.C. Edelstein, S.M. Finegold Wadsworth Anaerobic Bacteriology Manual 4th ed C.V. Mosby St. Louis
  5. Wilkens, T.D., S.L. Chalgren, F. Jimenez-Ulate, C.R. Drake Jr., J.L. Johnson 1976 Inhibition of Bacteroides fragilis on blood agar plates and reversal of inhibition by added hemin. J. Clin. Microbiol. 3:359-363.


CATALOGUE NUMBERS

Dehydrated media 500 g QB-39-3835
     
Enrichment Vitamin K-Hemin, 5ml 10/pkg 8751
     
Sheep Blood 50 ml 4245

 


 

 

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