m-TGE BROTH

Technical Data #39-4420a / 2013.07.21

m-TGE Broth is a non-selective broth medium used for the determination of bacterial counts by the membrane filter method. The ingredients are twice the concentration of TGEA Agar without agar.

 


 

FORMULA
in grams per liter purified filtered water

Casein Peptone

10,0

Beef Extract

6,0

Dextrose

2,0

pH 7,0 +/- 0,2 à 25º C

This approximate formula may be adjusted and/or enriched to obtain best results.


PRECAUTIONS
This medium is for IN VITRO diagnostic use only.


STORAGE
Store prepared media at 2-8º C protected from direct light and dehydrated powder, in a dry place in tightly-sealed containers at 2-25º C.


SIGN OF DETERIORATION
Media should not be used if the expiry date has passed. Prepared media should not be used if there are signs of contamination (shrinking, cracking, evaporation or discoloration). Do not use dehydrated media if it is caked.


DIRECTIONS
Suspend 18 g in 1000 ml of purified water. Heat with frequent agitation to dissolve. Dispense into culture tubes. Sterilize at 121º C for 15 minutes.


PROCEDURE
Bring prepared medium at room temperature before inoculation.

  1. Inoculate specimen as described in appropriate references.
  2. Incubate at 35º C for 24 hours.
  3. Examine for growth.


QUALITY CONTROL

Expected results after 24 hrs at 35ºC

Organisms

ATCC

Growth

Escherichia coli

25922

+

Staphylococcus aureus

25923

+


LIMITATIONS OF METHOD
This medium is only a part of the identification. Other tests may be required.


REFERENCES

  1. Mac Faddin, Jean F. 1985 Media for Isolation-Cultivation-Identification-Maintenance of Medical Bacteria. Vol. I, Baltimore, MD.: Williams & Wilkins.
  2. Compendium of methods for the microbiological examination of foods. A.P.H.A. Washington, DC, 1976
  3. Standards Methods for the examination of water and wastewater 16th ed. A.P.H.A. 1985 Washington, DC


CATALOGUE NUMBERS

Dehydrated media 500 g QB-39-4420

 


 

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