ENTERIC PATHOGEN TRANSPORT MEDIUM

Technical Data #EPTa / 2013.07.21

EPT Medium a semi-liquid medium is used for collection and transport of clinical specimens containing enteric pathogens especially the fastidious microorganisms as Campylobacter sp., Yersinia sp. Shigella sp., at room temperature. Increased pH, decreased agar content, and addition of phenol red permit maintenance of microorganism's viability during transport.

 


 

FORMULA
in grams per liter purified filtered water

Sodium Thioglycollate

1,5

Sodium Phosphate, dibasic

1,1

Potassium Phosphate, dibasic

0,2

Sodium Chloride

5,0

Calcium Chloride

0,09

Phenol Red

0,003

Agar

1,6

pH 8,4 +/- 0,2 à 25º C

This approximate formula may be adjusted and/or enriched to obtain best results.


PRECAUTIONS
This medium is for IN VITRO diagnostic use only.


STORAGE
Store prepared media at 2-25º C protected from direct light and dehydrated powder, in a dry place in tightly-sealed containers at 2-8º C.


SIGN OF DETERIORATION
Media should not be used if the expiry date has passed. Prepared media should not be used if there are signs of contamination (evaporation or discoloration). Do not use dehydrated media if it is caked.

 

DIRECTIONS
Suspend 9,5 g in 1000 ml of purified water. Heat with frequent agitation and boil for one minute. Sterilize at 121º C for 15 minutes. Cool to 45-50º C. Mix thouroughly and dispense into sterile vials or containers.


PROCEDURE

  1. Fill the vial till to the line with formed stool or liquid feces. Mix thouroughly and bring to the laboratory immediately.
  2. Subculture to appropriate selective and non selective culture media with EPT mixture. Streak for isolated colonies.
  3. Incubate at suggested temperature and as long as required.


QUALITY CONTROL

Subculture after 4 days transport at 25º C

Organisms

ATCC

Growth

Escherichia coli

25922

+

Shigella flexneri

12022

+

Yersinia enterocolitica

9610

+

Salmonella typhimurium

14028

+

Campylobacter jejuni

33291

+


LIMITATIONS OF METHOD
This is only a part of the identification. Others tests may be required.


REFERENCES

  1. Cary, S.G., M.H. Fusillo, C. Harkins 1965 Survival of Shigella and Salmonella in a new transport medium Am. J. Clin. Pathol 43:294
  2. Finegold, S.M., E.J. Baron 1986 Bailey and Scott's Diagnostic Microbiology 7th ed. C.V. Mosby St. Louis
  3. Lennette, E.H., Ballows, A., Hausler, W.J.Jr., and Shadomy, H.J. Manual of Clinical Microbiology. 4th ed. 1985 Washington D.C.: American society for Microbiology.
  4. Luechtefeld, M.W., W.L.L. Wang, M.J. Blaser, L.B. Reller 1981 Evaluation of transport and storage techniques for isolation of Campylobacter fetus subsp. jejuni from turkey cecal specimens J. Clin. Microbiol 13:438
  5. Mac Faddin, Jean F. 1985 Media for Isolation-Cultivation-Identification-Maintenance of Medical Bacteria. Vol. I, Baltimore, MD.: Williams & Wilkins.


CATALOGUE NUMBERS

Dehydrated media 500 g QB-39-1530
     
Prepared media    
Vials 15 ml 100/box EPT-100
  25/box EPT-25
Containers 45 ml 100/box EPT-100C

 


 

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