FORMALIN FIXATIVE FOR INTESTINAL PARASITES

Technical Data #FORMALINa / 2013.07.21

Formalin Fixative for intestinal parasites is used to preserve intestinal parasites for later examination. This solution which preserves parasites cysts, eggs and larvae may be used for concentrations procedures and for direct smears examinations.

 


 

FORMULA
in grams per liter purified filtered water

Sodium Phosphate, dibasic

0,763

Sodium Phosphate, monobasic

0,019

Formaldehyde, filtered

100,0 ml

Water, deionized

900,0

pH 7,3 +/- 0,2 à 25º C

This approximate formula may be adjusted and/or enriched to obtain best results.


PRECAUTIONS
This medium is for IN VITRO diagnostic use only.


STORAGE
Store prepared media at 25º C protected from direct light.


PROCEDURE FOR SPECIMEN COLLECTION
Collect specimens as previously standardized, following collection procedures. Bring to the laboratory as soon as possible

 

PROCEDURE
Wet Mount Examination

  1. On a slide, place a drop of Formalin-stool mixture and a drop of saline/iodine.
  2. Overlay with a coverglass.
  3. Examine microscopically for presence of parasites

Concentration Procedure
Follow concentration procedures as described in Para-Test Kit


LIMITATIONS OF METHOD

Inadequate fixation or too little/too much specimen may give false results. This is only a part of the identification. Others tests may be required.


REFERENCES

  1. Lennette, E.H., Ballows, A., Hausler, W.J.Jr., and Shadomy, H.J. Manual of Clinical Microbiology. 4th ed. 1985 Washington D.C.: American society for Microbiology.
  2. Yang, J., T. Scholten 1977 A fixative for intestinal parasites permitting the use of concentration and permanent staining procedures Am. J. Clin. Pathol 67:300-304


CATALOGUE NUMBERS

FORMALIN    
Vial 15 ml 100/box FN-100
Tube 3 ml 500/cs FO-03
Container 20 ml 150/cs FO-20
Container 45 ml 100/box FO-45

 


 

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